diff --git a/.changeset/two-ads-bathe.md b/.changeset/two-ads-bathe.md
new file mode 100644
index 0000000000..beb3301460
--- /dev/null
+++ b/.changeset/two-ads-bathe.md
@@ -0,0 +1,5 @@
+---
+"astro": minor
+---
+
+Adds support for page mutations to the audits in the dev toolbar. Astro will now rerun the audits whenever elements are added or deleted from the page.
diff --git a/packages/astro/e2e/dev-toolbar-audits.test.js b/packages/astro/e2e/dev-toolbar-audits.test.js
index 2195aa9f95..cbb89ab728 100644
--- a/packages/astro/e2e/dev-toolbar-audits.test.js
+++ b/packages/astro/e2e/dev-toolbar-audits.test.js
@@ -44,6 +44,115 @@ test.describe('Dev Toolbar - Audits', () => {
 		await appButton.click();
 	});
 
+	test('can handle mutations', async ({ page, astro }) => {
+		await page.goto(astro.resolveUrl('/audits-mutations'));
+
+		const toolbar = page.locator('astro-dev-toolbar');
+		const appButton = toolbar.locator('button[data-app-id="astro:audit"]');
+		await appButton.click();
+
+		const auditCanvas = toolbar.locator('astro-dev-toolbar-app-canvas[data-app-id="astro:audit"]');
+		const auditHighlights = auditCanvas.locator('astro-dev-toolbar-highlight');
+		await expect(auditHighlights).toHaveCount(1);
+
+		await page.click('body');
+
+		const badButton = page.locator('#bad-button');
+
+		let consolePromise = page.waitForEvent('console');
+		await badButton.click();
+		await consolePromise;
+
+		await appButton.click();
+		await expect(auditHighlights).toHaveCount(2);
+	});
+
+	test('multiple changes only result in one audit update', async ({ page, astro }) => {
+		await page.goto(astro.resolveUrl('/'));
+
+		await page.evaluate(() => {
+			localStorage.setItem(
+				'astro:dev-toolbar:settings',
+				JSON.stringify({
+					verbose: true,
+				})
+			);
+		});
+
+		await page.goto(astro.resolveUrl('/audits-mutations'));
+
+		let logs = [];
+		page.on('console', (msg) => {
+			logs.push(msg.text());
+		});
+
+		const badButton = page.locator('#bad-button');
+
+		let consolePromise = page.waitForEvent('console', (msg) =>
+			msg.text().includes('Rerunning audit lints')
+		);
+		await badButton.click({ clickCount: 5 });
+		await consolePromise;
+
+		await page.click('body');
+
+		expect(
+			logs.filter((log) => log.includes('Rerunning audit lints because the DOM has been updated'))
+				.length === 1
+		).toBe(true);
+	});
+
+	test('handle mutations properly during view transitions', async ({ page, astro }) => {
+		await page.goto(astro.resolveUrl('/'));
+
+		await page.evaluate(() => {
+			localStorage.setItem(
+				'astro:dev-toolbar:settings',
+				JSON.stringify({
+					verbose: true,
+				})
+			);
+		});
+
+		await page.goto(astro.resolveUrl('/audits-mutations'));
+
+		let logs = [];
+		page.on('console', (msg) => {
+			logs.push(msg.text());
+		});
+
+		const linkToOtherPage = page.locator('#link-to-2');
+		let consolePromise = page.waitForEvent('console');
+		await linkToOtherPage.click();
+		await consolePromise;
+
+		const toolbar = page.locator('astro-dev-toolbar');
+		const appButton = toolbar.locator('button[data-app-id="astro:audit"]');
+
+		await appButton.click();
+
+		const auditCanvas = toolbar.locator('astro-dev-toolbar-app-canvas[data-app-id="astro:audit"]');
+		const auditHighlights = auditCanvas.locator('astro-dev-toolbar-highlight');
+		await expect(auditHighlights).toHaveCount(1);
+
+		await page.click('body');
+
+		const badButton = page.locator('#bad-button-2');
+
+		consolePromise = page.waitForEvent('console');
+		await badButton.click();
+		await consolePromise;
+
+		await appButton.click();
+		await expect(auditHighlights).toHaveCount(2);
+
+		// Make sure we only reran audits once
+		expect(
+			logs.filter((log) => log.includes('Rerunning audit lints because the DOM has been updated'))
+				.length === 1
+		).toBe(true);
+	});
+
 	test('does not warn for non-interactive element', async ({ page, astro }) => {
 		await page.goto(astro.resolveUrl('/a11y-exceptions'));
 
diff --git a/packages/astro/e2e/dev-toolbar.test.js b/packages/astro/e2e/dev-toolbar.test.js
index b2e6242b47..49472fbb3b 100644
--- a/packages/astro/e2e/dev-toolbar.test.js
+++ b/packages/astro/e2e/dev-toolbar.test.js
@@ -272,7 +272,6 @@ test.describe('Dev Toolbar', () => {
 		await appButton.click();
 
 		const myAppCanvas = toolbar.locator('astro-dev-toolbar-app-canvas[data-app-id="my-plugin"]');
-		console.log(await myAppCanvas.innerHTML());
 		const myAppWindow = myAppCanvas.locator('astro-dev-toolbar-window');
 		await expect(myAppWindow).toHaveCount(1);
 		await expect(myAppWindow).toBeVisible();
diff --git a/packages/astro/e2e/fixtures/dev-toolbar/src/pages/audits-mutations-2.astro b/packages/astro/e2e/fixtures/dev-toolbar/src/pages/audits-mutations-2.astro
new file mode 100644
index 0000000000..e1c95e3e4d
--- /dev/null
+++ b/packages/astro/e2e/fixtures/dev-toolbar/src/pages/audits-mutations-2.astro
@@ -0,0 +1,29 @@
+---
+import Layout from "../layout/Layout.astro";
+---
+
+<Layout>
+<button id="bad-button-2">Click me to add an image that is missing an alt!</button>
+<a id="link-to-1" href="/audits-mutations">Go to Mutations 1</a>
+
+<br /><br /><br />
+<img src="" width="100" height="100" />
+
+<script>
+	document.addEventListener('astro:page-load', () => {
+		const badButton = document.getElementById('bad-button-2');
+		if (!badButton) return;
+
+		badButton.addEventListener('click', clickHandler);
+
+		function clickHandler() {
+			const img = document.createElement('img');
+			img.width = 100;
+			img.height = 100;
+
+			document.body.appendChild(img);
+			console.log("Image added to the page")
+		}
+	})
+</script>
+</Layout>
diff --git a/packages/astro/e2e/fixtures/dev-toolbar/src/pages/audits-mutations.astro b/packages/astro/e2e/fixtures/dev-toolbar/src/pages/audits-mutations.astro
new file mode 100644
index 0000000000..1c4950a2ff
--- /dev/null
+++ b/packages/astro/e2e/fixtures/dev-toolbar/src/pages/audits-mutations.astro
@@ -0,0 +1,28 @@
+---
+import Layout from "../layout/Layout.astro";
+---
+
+<Layout>
+<button id="bad-button">Click me to add an image that is missing an alt!</button>
+<a id="link-to-2" href="/audits-mutations-2">Go to Mutations 2</a>
+
+<img src="" width="100" height="100" />
+
+<script>
+	document.addEventListener('astro:page-load', () => {
+		const badButton = document.getElementById('bad-button');
+		if (!badButton) return;
+
+		badButton.addEventListener('click', clickHandler);
+
+		function clickHandler() {
+			const img = document.createElement('img');
+			img.width = 100;
+			img.height = 100;
+
+			document.body.appendChild(img);
+			console.log("Image added to the page")
+		}
+	})
+</script>
+</Layout>
diff --git a/packages/astro/src/runtime/client/dev-toolbar/apps/audit/index.ts b/packages/astro/src/runtime/client/dev-toolbar/apps/audit/index.ts
index e07e6c6ac8..1e7e3009c2 100644
--- a/packages/astro/src/runtime/client/dev-toolbar/apps/audit/index.ts
+++ b/packages/astro/src/runtime/client/dev-toolbar/apps/audit/index.ts
@@ -10,6 +10,7 @@ import {
 import { closeOnOutsideClick, createWindowElement } from '../utils/window.js';
 import { a11y } from './a11y.js';
 import { perf } from './perf.js';
+import { settings } from '../../settings.js';
 
 const icon =
 	'<svg xmlns="http://www.w3.org/2000/svg" fill="none" viewBox="0 1 20 16"><path fill="#fff" d="M.6 2A1.1 1.1 0 0 1 1.7.9h16.6a1.1 1.1 0 1 1 0 2.2H1.6A1.1 1.1 0 0 1 .8 2Zm1.1 7.1h6a1.1 1.1 0 0 0 0-2.2h-6a1.1 1.1 0 0 0 0 2.2ZM9.3 13H1.8a1.1 1.1 0 1 0 0 2.2h7.5a1.1 1.1 0 1 0 0-2.2Zm11.3 1.9a1.1 1.1 0 0 1-1.5 0l-1.7-1.7a4.1 4.1 0 1 1 1.6-1.6l1.6 1.7a1.1 1.1 0 0 1 0 1.6Zm-5.3-3.4a1.9 1.9 0 1 0 0-3.8 1.9 1.9 0 0 0 0 3.8Z"/></svg>';
@@ -69,8 +70,51 @@ export default {
 
 		await lint();
 
-		document.addEventListener('astro:after-swap', async () => lint());
-		document.addEventListener('astro:page-load', async () => refreshLintPositions);
+		let mutationDebounce: ReturnType<typeof setTimeout>;
+		const observer = new MutationObserver(() => {
+			// We don't want to rerun the audit lints on every single mutation, so we'll debounce it.
+			if (mutationDebounce) {
+				clearTimeout(mutationDebounce);
+			}
+
+			mutationDebounce = setTimeout(() => {
+				settings.logger.verboseLog('Rerunning audit lints because the DOM has been updated.');
+
+				// Even though we're ready to run the lints, we'll wait for the next idle period to do so, as it is less likely
+				// to interfere with any other work the browser is doing post-mutation. For instance, the page or the user might
+				// be interacting with the newly added elements, or the browser might be doing some work (layout, paint, etc.)
+				if ('requestIdleCallback' in window) {
+					window.requestIdleCallback(
+						async () => {
+							lint();
+						},
+						{ timeout: 300 }
+					);
+				} else {
+					// Fallback for old versions of Safari, we'll assume that things are less likely to be busy after 150ms.
+					setTimeout(() => {
+						lint();
+					}, 150);
+				}
+			}, 250);
+		});
+
+		setupObserver();
+
+		document.addEventListener('astro:before-preparation', () => {
+			observer.disconnect();
+		});
+		document.addEventListener('astro:after-swap', async () => {
+			lint();
+		});
+		document.addEventListener('astro:page-load', async () => {
+			refreshLintPositions();
+
+			// HACK: View transitions add a route announcer after this event, so we need to wait for it to be added
+			setTimeout(() => {
+				setupObserver();
+			}, 100);
+		});
 
 		closeOnOutsideClick(eventTarget);
 
@@ -380,5 +424,12 @@ export default {
 				.replace(/"/g, '&quot;')
 				.replace(/'/g, '&#039;');
 		}
+
+		function setupObserver() {
+			observer.observe(document.body, {
+				childList: true,
+				subtree: true,
+			});
+		}
 	},
 } satisfies DevToolbarApp;